Abstract The microbiota that colonize the human gut and other tissues are dynamic, varying both in composition and functional state between individuals and over time.Gene expression measurements can provide insights into microbiome composition and function.However, efficient and unbiased removal of microbial ribosomal RNA (rRNA) presents a barrier
ORM1-like 3 (ORMDL3) has strong genetic linkage to childhood onset asthma.To determine whether ORMDL3 selective expression in airway smooth muscle (ASM) influences ASM function, we used Cre-loxP door mat techniques to generate transgenic mice (hORMDL3Myh11eGFP-cre), which express human ORMDL3 selectively in smooth muscle cells.In vitro studies of A
The fundamental role of Vitamin D has been long known in regulating calcium homeostasis and bone metabolism.An increased contribution of Vitamin D was recently described in association with a lower incidence of Rheumatoid Arthritis (RA).This must not be surprising, as the immunomodulating effects of Vitamin D are clear, which have been attributed p
Environmental isotope tracers were applied in the Upper Crocodile River Basin, Johannesburg, South Africa, to understand Toast Tongs the groundwater recharge conditions, flow mechanisms and interactions between surface and subsurface water.Stable isotope analysis indicated that recharge into the fractured quartzite aquifer occurs through direct mec